CryoBioPhysica.com, pISep Advanced Protein Purification Technology

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CryBioPhysica. Inc. presents pISep pH Gradient Maker
pISep Chromatographic Software Applications for Formation of User Controlled
pH Gradients and Simple Scale up procedures

What are the benefits and what I can do?

You can use pISep pH Gradient Maker software to quickly and easily calculate the chromatographic parameters - the changes of %B (and %A) vs. column volumes to program your FPLC or HPLC in order to develop ascending or descending multi-step linear pH gradients with user defined slopes, for any starting and ending pH in the range pH 2.4 to pH 10.7 when using pISep buffers.
Alternatively, you can use pISep pH Gradient Maker software to quickly and easily calculate the changes of %B (and %A) vs. column volumes to program your FPLC or HPLC in order to generate ascending or descending multi-step nonlinear or mixed linear and nonlinear pH gradients with arbitrary curve shapes – concave, convex or stepwise when using pISep buffers.
pISep pH Gradient Maker software gives you the flexibility to create your own coefficients for developing pH gradients with pISep buffers in presence of non-ionic or zwitterionic detergents of your choice allowing you to customize the purification of your specific proteins.
Use the exclusive, unmatched flexibility of pISep pH Gradient Maker software to specifically optimize your pIsep chromatographic protein separation or your pIsep fractionation of other charged biological molecules.
Use pISep pH Gradient Maker software to calculate the necessary parameters to perform a simple chromatographic scale-up of an optimized analytical pISep purification protocol to a semi-preparative or preparative purification protocol using the same ion exchanger packed in a semi-preparative or preparative column.
Use pISep pH Gradient Maker software to calculate the necessary parameters to perform a simple chromatographic scale-up of an optimized analytical pISep purification protocol to a semi-preparative or preparative purification protocol using the same ion exchanger packed in a semi-preparative or preparative column.
Remember no more desalting, if any anionic or/and cationic exchange step with salt elution in your present chromatographic protocol is replaced with an anionic or cationic pISep step, you will save time and money while gaining both better resolution quality and better efficiency of your separation.